The CellSearch® Epithelial Cell Kit enables the immunomagnetic selection, identification and enumeration of circulating epithelial cells in peripheral blood. The kit contains a ferrofluid-based capture reagent and immunofluorescent staining reagents. The ferrofluid reagent consists of nanoparticles with a magnetic core surrounded by a polymeric layer coated with antibodies targeting the EpCAM antigen for capturing circulating epithelial cells. After immunomagnetic capture and enrichment, fluorescent staining reagents are added for identification and enumeration of circulating epithelial cells. Anti-CKPE is specific for the intracellular protein cytokeratin (specific for epithelial cells), DAPI stains the cell nucleus and anti-CD45-APC is specific for leukocytes.
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procedures. Last updated on: 22 April 2013